SPADE™ is written in the multi-platform language Python, and has dependencies that are also free and multi-platform. These must be downloaded and installed for non-Windows users, as described below. Please report installation problems in the Forums.
To launch SPADE from the source package, double-click SPADE.py in the downloaded, unzipped directory. Windows users can run SPADE from the Desktop or Quick-Launch icons. Note that the first time SPADE is loaded it will take 1-2 minutes to compile many .pyc files. The molecular viewer and database manager are immediately available for use. Use the menu command File>>Download a PDB to query the RCSB database by accession name. Try 1otc for instance, the dimeric Telomere End-Binding Protein. Once downloaded, the given project title appears in the 'systems' listings. Select it, and launch its molecular viewer by pressing the button on its right hand side.
To download applications (which typically provide a demo project), use the menu command Downloads>>application, and select from the available listings. Once an application packet has downloaded, the new application and new demo system automatically appear in the Control Panel listings. To open a molecular viewer, click on a Molecular System and then the launch button to its right. Then launch the associated application by clicking its title in the top pane of the SPADE control panel. The application launches into a new tab in the molecular viewer window and you are free to explore its user interface.
Installers for SPADE and for the stand-alone Molecular Viewer are now available at the SourceForge download site. To run SequencePad, install clustalw to the SPADE main directory (typically C:/Program Files/SPADE). To run RAVE, also install matplotlib and Python Imaging Library, as described below
Windows Developers There are several dependencies required for running SPADE without the executable installer. First, download the zip file for the most recent SPADE version from the SourceForge Files page. Then go to http://www.lfd.uci.edu/~gohlke/pythonlibs/ to obtain and install the following packages, as appropriate for your operating system and the Python 2.7 that SPADE currently runs on: NumPy, SciPy, MatPlotLib, BioPython, Pillow, VTK (not VTK-Qt) On older systems I used to have to then copy vtkRenderingPythonTkWidgets.dll from C:\Python27\Lib\site-packages\vtk to my System directory (e.g. C:\Windows\System32), but my current installation did not require that. That should be it. If there are any issues please let us know in the forums.
Required Core Packages:
VTK Home About Download a powerful graphics library. Note that VTK does not automatically work with Python. A fix for this will be available soon. For now please use Enthon, if you are in a degree-granting institution. Advice for non-academic Windows installation is available here.
SPADE Download code is hosted by the SourceForge project. Decompress the downloaded Zip file with i.e. 7-zip.
Requirements for SequencePad:
Clustalw Home About Download Note that ClustalW may not be free for commercial use. Please read the licence here. Place the executable (clustalw2.exe or clustalw2) into the SPADE directory.
Requirements for RAVE: